Product details Animal Tissue Total RNA Purification Kit (Spin Column) [Product Code] 208111 [Packing Specification] 50 reactions [Introduction] The Total RNA Extraction Kit for Animal Tissue / Cell (hereinafter referred to as the “Product”) adopts a unique lysis-buffer system without the application of β-mercaptoethanol, Dithiothreitol (DTT) or other toxic reagents, which can effectively extract total RNA from various animal tissues or cells. The centrifugal spin columns adopt silica as a substrate material, which enables the centrifugal spin columns to efficiently adsorb RNA and remove impurity proteins and other organic compounds from cells. Therefore, the extracted total RNA has high yield, and sound purity, with stable and reliable quality, which can be used for various downstream experiments such as RT-PCR, Real Time RT-PCR and molecular cloning. [Major Components] Component Content Buffer ZRL 22 mL Buffer WA 23 mL Buffer WB 14 mL RNase-Free Water 7 mL Proteinase K 1.04 mL Spin Column 50 units Collection tube 50 units [Storage Conditions & Shelf Life] Transported at room temperature and stored at room temperature away from light. The shelf life is 12 months. Production Date: Refer to the label. Expiration Date: Refer to the label. [Necessary Devices and Equipment before Use] 1. RNase-free centrifuge tubes (1.5 mL); 3 samples 2. Absolute ethyl alcohol 3. Single-channel Pipettes and tips: 50 μL, 200 μL and 1,000 μL 4. Block heater (or water bath): 37°C and 56°C 5. Centrifuge (speed: 12,000 rpm) Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.